| ORIGINAL ARTICLE |
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| Year : 2017 | Volume
: 1
| Issue : 2 | Page : 63-68 |
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Expression of cellular FLICE-like inhibitory protein in granulosa cells in In Vitro fertilization patients with advanced endometriosis
Jian-Ping Zhang, Mei-Qin Yan, Yao-Qin Wang, Fang Yang, Juan Liu, Xue-Qing Wu
Human Assisted Reproduction Center, Shanxi Women and Children's Hospital, Taiyuan 030013, China
Correspondence Address:
Xue-Qing Wu
Human Assisted Reproduction Center, Shanxi Women and Children's Hospital, Taiyuan 030013
China
 Source of Support: None, Conflict of Interest: None
DOI: 10.4103/2096-2924.216861
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Background: This study evaluated the expression of cellular FLICE-like inhibitory protein (cFLIP) in granulosa cells (GCs) obtained from in vitro fertilization-embryo transfer patients with advanced endometriosis.
Methods: A total of 267 patients with advanced endometriosis were enrolled in this study. They were divided into clinical pregnancy group (n = 114) and nonpregnancy group (n = 153). The expressions of cFLIP in mRNA and protein level were measured by real-time polymerase chain reaction (RT-PCR) and Western blotting. The related factors on the clinical pregnancy were analyzed using logistic regression analysis. Coefficients of correlation were calculated using the nonparametric rho-Spearman test.
Results: The number of oocytes retrieved, fertilization rate, and cleavage rate were significantly and independently related with clinical pregnancy (P < 0.05). RT-PCR and Western blotting analysis showed that the expressions of cFLP in mRNA and protein level were significantly higher in the clinical pregnancy group than in nonpregnancy group (P < 0.05). cFLIP had a significantly positive correlation with the number of oocytes retrieved (P < 0.05) and no correlation with fertilization rate and cleavage rate (P > 0.05).
Conclusion: Higher expression of cFLIP increased the pregnancy rate in women with advanced endometriosis.
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