Reproductive and Developmental Medicine

ORIGINAL ARTICLE
Year
: 2021  |  Volume : 5  |  Issue : 4  |  Page : 193--198

Age-cumulative effect of REC8 reduction on meiotic chromosome segregation errors in mice


Li-Yuan Tian1, Ling Zhang1, Cheng-Qiu Tao2, Xiao-Qi Lin2, Feng Zhang3, Bin Zhang1 
1 Department of Obstetrics, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, China
2 Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai 200011, China
3 Department of Obstetrics, Obstetrics and Gynecology Hospital of Fudan University; Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai 200011, China

Correspondence Address:
Bin Zhang
Department of Obstetrics, Obstetrics and Gynecology Hospital of Fudan University, 419 Fangxie Road, Shanghai 200011
China

Objective: This study aimed to explore the relationship between cohesin subunit REC8 reduction and meiosis chromosome segregation errors in the ovary. Methods: Rec8+/− mice were generated using CRIPSR/Cas9 gene editing. The association between age and REC8 expression levels in the ovary was determined by Western blotting. Chromosome segregation errors were investigated by immunofluorescence imaging of superovulated oocytes. Wild-type and Rec8+/− female mice at 5, 8, 20, 36, and 40 weeks were used to evaluate ovarian reserve by ovarian clearing and immunolabeling. Results: Ovary REC8 expression levels gradually decreased with age, while chromosome segregation errors increased with age. Segregation errors were more common in Rec8+/− mice, suggesting an association with REC8 expression. The ovarian reserve capacity decreased significantly with age. The ovarian reserve in Rec8+/− mice was inferior to that of age-matched wild-type mice, indicating important roles of age and REC8 levels in the ovarian reserve. Conclusions: REC8 reduction has an age-cumulative effect on meiotic chromosome segregation errors in mouse ovaries. Rec8 haploinsufficiency poses a major challenge in generating normal and reproductive oocytes in aging mice.


How to cite this article:
Tian LY, Zhang L, Tao CQ, Lin XQ, Zhang F, Zhang B. Age-cumulative effect of REC8 reduction on meiotic chromosome segregation errors in mice.Reprod Dev Med 2021;5:193-198


How to cite this URL:
Tian LY, Zhang L, Tao CQ, Lin XQ, Zhang F, Zhang B. Age-cumulative effect of REC8 reduction on meiotic chromosome segregation errors in mice. Reprod Dev Med [serial online] 2021 [cited 2022 Jun 30 ];5:193-198
Available from: https://www.repdevmed.org/article.asp?issn=2096-2924;year=2021;volume=5;issue=4;spage=193;epage=198;aulast=Tian;type=0